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. 2017 May 24;8(29):47998–48011. doi: 10.18632/oncotarget.18208

Figure 5. EphA2 regulate EMT markers.

Figure 5

(A) The mRNA expression levels of three EMT markers (E-cadherin, N-cadherin, and Snail) in SGC-7901/L-OHP cells were assayed by quantitative real-time PCR. 18S rRNA was used as the internal control (ctrl). Compared with ‘the blank’ group (*P < 0.05). (B and C) Protein levels of the three EMT markers in were assayed by Western blotting; GAPDH was used as the internal control (Ctrl). Relative protein accumulation of proteins in cells compared with the ‘blank ctrl’ group is indicated (*P < 0.05). (D) Immunofluorescence reveals the presence of three the EMT markers. Targeted proteins were stained green. (E) Post-transfection of SGC-7901/L-OHP cells with EphA2 siRNA. Changes in cell phenotype are shown as the loss of mesenchymal properties and acquisition of epithelial characteristics.