Skip to main content
. 2017 May 29;8(29):48098–48109. doi: 10.18632/oncotarget.18259

Figure 1. Serum levels of estradiol and expression of Rcan2 mRNA in OVX mice.

Figure 1

(A) Serum levels of estradiol in sham-operated (sham) and ovariectomized (OVX) mice. Statistics were performed using one-way ANOVA, and individual group differences presented here were measured using Bonferroni's correction. **: p <0.01. (B) Representative images of uteri in sham mice and OVX mice. (C) Expression levels of Rcan2-1 and Rcan2-3 mRNA in hypothalamus. Total RNA was isolated from hypothalami of sham and OVX WT mice and subjected to quantitative real-time PCR analysis using primers specific for Rcan2-1, Rcan2-3, and β-actin (internal standard). mRNA expression levels are expressed relative to those of sham WT mice. The number of mice (n) used in the experiments was indicated directly in each panel of figures. All values are given as means ± SEM.