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. 2017 Jul 5;7(13):e2388. doi: 10.21769/BioProtoc.2388

Figure 3. Zeiss AxioZoom.V16 set up for optogenetic dose response and blue light exposure diagram.

Figure 3.

A. Optogenetic dose response set up in a brightly lit room for demonstration purpose, but conduct the assays in a dimly lit room. 1. Zeiss AxioZoom.V16, 2. Glass plate, 3. Blue light, 4. Aperture control and 5. Dark field illumination. B. Sample blue light on/off time course; C. Image stills of larvae expressing ChETA in class III md neurons being exposed 100% or 66% max blue light. 100% illumination elicits CT behavior (consistent with noxious cold evoked behavior), whereas 66% illumination elicits HW behavior (consistent with gentle touch evoked behavior).