Solution	Component
1x PBS	Dilute 10x PBS with autoclaved water
NSC medium	2 mL 50x B27
	1 mL 100x N2
	1 mL 200 mM L-glutamine
	1 mL 100x Pen/Strep
	FGF-basic (human bFGF) (Stock solution 25 ng/mL in Neurobasal medium): 20 ng/mL or 2 mg total for 100 mL media
	EGF (Stock solution 25 ng/mL in Neurobasal medium): 20 ng/mL or 2 mg total for 100 mL media
	95 mL basal medium
Human FGF-basic (human bFGF)	1 mL basal medium to commercial human bFGF 25 μg bottle
	Store in sterile microtubes in -20 °C or lower. Use a single 80 μL aliquot or 2 μg/100 mL NSC media.
EGF	Add 8 mL basal medium to commercial EGF 20 μg bottle
	Store as sterile microtubes in -20 °C or lower. Use a single 80 μg aliquot/100 mL media.
Stopping medium	1 mL FBS
	75 U Dnase I (75 U/uL in PBS)
	9 mL PBS
Serum medium	1 mL FBS
	9 mL Basal medium
Freezing medium	4 mL DMSO
	24 mL basal medium
	12 mL 30% FBS
Differentiation medium	90 mL basal medium
	5 mL 5% FBS
	1 mL 100x Pen/Strep
	1 mL 200 mM L-glutamine
	1 mL 100x N2
	2 mL 50x B27
	FGF-basic (human bFGF) (Stock solution of 25 ng/mL in Neurobasal medium): 10 ng/mL or 1 mg total for 100 mL
Starvation medium	95 mL basal medium
	1 mL 100x Pen/Strep
	1 mL 200 mM L-glutamine
	1 mL 100x N2
	2 mL 50x B27