Figure 5. Overexpression of either ZNF131 or HAUS5 kd cause increased MT-nucleation along the mitotic spindle and at spindle poles.

Quantification of α-tubulin staining at spindle pole and along spindle was performed 48hrs after selection for LV-ZNF131 and LV-HAUS5 in GSC-0131 cells as detailed in methods (n = 30 for each group) (using devolved 3D images aquired by a Deltavision imaging station). *indicates p-value < .001, as compared to control values.