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. 2017 Jun 1;8(30):48563–48574. doi: 10.18632/oncotarget.18335

Figure 5. High-fat diet induces changes in immune composition of the liver.

Figure 5

A. Gating strategy for liver lymphoid cell populations. B cells (CD19+CD3−), NK cells (NK1.1+CD3−), NKT cells (NK1.1+CD3+), CD8 T cells (CD3+NK1.1−CD8+) and CD4 T cells (CD3+NK1.1−CD4+) were first gated for singlets and on a viability marker. B. Absolute numbers of the various lymphoid immune cells in livers from WT and IL-33 KO mice fed a SD or HFD. C. Gating strategy for liver myeloid cell populations. Granulocytes (CD11bhighGr1+F4/80−) and macrophages (CD11bhighGr1−F4/80+) were first gated for singlets and on a viability marker. D. Absolute numbers of the various myeloid immune cells in livers from WT and IL-33 KO mice fed a SD or a HFD. Statistical analysis of the data was performed using the non-parametric Mann-Whitney U-test. Differences were considered to be significant for p < 0.05 and are indicated as follows: *p < 0.05, **p < 0.01.