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. 2017 Apr 6;8(30):48983–48995. doi: 10.18632/oncotarget.16904

Figure 5. Glutamine withdrawal increases the number of dead cells in MYC amplified SCLC cells in a HIF-independent fashion.

Figure 5

The total number of viable and dead cells at 21% or 1% oxygen was counted after 72 hours. The arrows indicate the number of cells seeded at day 0. (A) The MYC amplified SCLC cell lines U-1906, transduced with shRNA (sh-H1) against HIF1A or a non-targeting control (sh-C), were grown in RPMI medium with (2 mM) or without glutamine (−Gln). (D and F) The non-MYC amplified NCI-H345 SCLC cells and SH-SY5Y neuroblastoma cells were cultured with or without glutamine. (B, E, and G) The percentage of dead cells compared to total number of cells is also presented. (C) ATP levels were determined by a luciferin-luciferase based assay. Data are mean from two to four experiments; error bars show the standard deviation. All statistical analyses were performed using 2-tailed unpaired Student's t test, * indicates p < 0.05, ** < 0.01, *** < 0.001.