Skip to main content
. 2017 Apr 13;8(30):49093–49109. doi: 10.18632/oncotarget.17095

Figure 8. The rescued experiment of carcinogenesis effect of the JMJD2A in Hep3B cell lines transfected with pCMV6-AC-GFP, pCMV6-AC-JMJD2A (GFP-JMJD2A), pCMV6-AC-GFP-JMJD2A plus pcDNA3.1-P21WAF1/Cip1, pCMV6-AC-JMJD2A puls pGFP-V-RS-Pim1.

Figure 8

(A) Western blotting analysis with anti-JMJD2A, and anti-P21WAF1/Cip1 and anti-Pim1. β-actin served as internal control. (B) Cells growth assay using CCK8. Each value was presented as mean ± standard error of the mean (SEM).**P < 0.01. (C) Cell BrdU staining assay. Each value was presented as mean ± standard error of the mean (SEM).**P < 0.01. (D) Cells soft agar colony formation assay. (E) Tumorigenesis test in vivo (a) The wet weight of each tumor was determined for each mouse. Each value was presented as mean ± standard error of the mean (SEM).**P < 0.01. (b) The appearance time of each tumor was determined for each mouse. Each value was presented as mean ± standard error of the mean (SEM).**P < 0.01. (c) A portion of each tumor was fixed in 4% paraformaldehyde and embedded in paraffin for PCNA staining (DAB stainning, original magnification×100).