Figure 5. As₂O₃ promotes cell death in high ERK8-expressing lung cancers.

Dose- and time-dependent cytotoxicity of As₂O₃ towards H1299 cells. H1299 cells were sham-exposed or exposed to 5-20 μM of As₂O₃ for 36 h or 5 μM As₂O₃ for 12-48 h. (A) cell morphology of H1299 cells under light microscope. (B) The corresponding western blot of (A), cells were lysed and protein extracts were subjected to western blot analyses using antibodies against Bax, Caspase-9, and Caspase-3. After development, the membranes were stripped and reprobed with β-actin to monitor the loading difference. (C) Stable shMock and shERK8 H1299 cells were sham-exposed or exposed to 5 μM As₂O₃ for 48 h, the percentage of cell viability was determined by MTS assay. *A significant difference of P < 0.05. (D) The corresponding western blot of (C) for the determination of marker proteins involved in apoptosis. The data are representative of three independent experiments.