Figure 3. Contribution of glucose/glutamine/glutamate anaplerosis and acetate influx to TCA cycle metabolism in IDH1^WT versus IDH1^MUT glioma.

Increased glucose/glutamate anaplerosis in IDH1^MUT glioma and high contribution of acetate in IDH^WT glioma metabolism. (A) Gene expression levels of metabolic enzymes involved in glucose anaplerosis, glutaminolysis and acetate metabolism in IDH1^WT (n = 399) and IDH1^MUT (n = 112) LGG and IDH1^WT (n = 157) and IDH1^MUT (n = 9) glioblastoma. Relative mRNA expression levels are shown for IDH1^WT (blue) and IDH1^MUT (red). (B) BCAT1 expression and methylation according to IDH1^MUT status (blue: IDH1^WT, red: IDH1^MUT). Abbreviations: PC, pyruvate carboxylase; GLUD, glutamate dehydrogenase; GLS, glutaminase; BCAT, branched-chain amino acid transferase; GS, glutamine synthetase; ACSS, acetyl-CoA synthetase.