Skip to main content
. 2017 Apr 27;8(30):49224–49237. doi: 10.18632/oncotarget.17480

Figure 5. Block of β1 integrin and JNK signaling enhances radiation induced DSB by chromatin modification.

Figure 5

(A) Western blot analysis of indicated proteins from whole cell lysates of U343MG and GS-8 cells treated with AIIB2/SP600125 (EC10) or control IgG/DMSO without and with X-ray irradiation (6 Gy). Fold changes are calculated by normalization to β-actin and IgG/DMSO controls according to representative blots. (B) Immunofluorescence analysis of nuclei with 53BP1-positive foci after indicated treatments. Scale bar, 10 μm. (C) Quantification of the number of DSB per cell at the indicated time points after treatment with AIIB2/SP600125 (EC10) or control IgG/DMSO without and with X-ray irradiation. (D) Basal surviving fraction of U343MG cells upon treatment with AIIB2/SP600125 (EC10), LBH589 (EC50) or a combination thereof compared to control treatment (IgG/DMSO). (E) Clonogenic radiation survival of U343MG cells treated as described in (H). (C–E) Results are mean +/− SEM (n = 3, t-test).