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. 2017 Apr 27;8(30):49238–49252. doi: 10.18632/oncotarget.17493

Figure 2. BMX promoted the proliferation of cervical carcinoma cells in vitro.

Figure 2

(A) The expression of BMX in cervical cancer cell lines was detected with a western blotting assay, and the quantitative analysis using Quantity One software is shown. (B) HeLa cells were treated with DMSO as control, 13 μM and 26 μM BMX-IN-1, and the expression of BMX was determined using a western blotting. (C) Treated HeLa cells with DMSO, and 26 μM BMX-IN-1, flow cytometry analysis was used to assess the cell proliferation with Brdu incorporation, p < 0.001. (D) SiHa cells were treated with DMSO, 6.5 μM and 13 μM BMX-IN-1, and the expression of BMX was determined. (E) Treated SiHa cells with DMSO, and 13 μM BMX-IN-1, the cell proliferation with Brdu incorporation was assessed, p < 0.001. (F) A western blotting assay was used to detect the expression of BMX in TALEN-mediated HeLa BMX-knockdown clones. (G) Growth curves and (H) flow cytometry analysis (p < 0.05) were used to assess the proliferation of HeLa-wt/BMX+/− cells. (I) A western blotting assay was used to detect the expression of BMX in shRNA-mediated SiHa BMX-knockdown clones. (J) Growth curves and (K) flow cytometry analysis (p < 0.05) were used to assess the proliferation of SiHa-shGFP/shBMX cells. (L) A western blotting assay was used to detect the expression of BMX in C-33A BMX-overexpressing clones (transfected with AcGFP or BMX-overexpression plasmid). (M) Growth curves and (N) flow cytometry analysis (p < 0.05) were used to assess the proliferation and viability of C-33A-AcGFP/BMX-overexpressing cells. Values are shown as the mean ± SEM from three independent experiments (t-test, *p < 0.05, **p < 0.01, ***p < 0.001 vs the corresponding control).