Figure 3. Expression of pathway-specific genes after IL-16 knockdown.

Quantitative RT-PCR assays were used to validate changes in mRNA expression in RPMI-8226 cells treated with IL-16-specific shRNA over cells expressing control shRNA. Genes are identified as pro-proliferative and anti-proliferative according to their respective gene ontology annotation [45]. Genes are clustered into the three major molecular pathways (A) PI3K, (B) NFκB, and (C) MAPK.