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. 2017 May 8;8(30):49451–49469. doi: 10.18632/oncotarget.17706

Figure 8. miR-29a-3p, miR-660-5p and miR494-3p overexpression in HSPCs isolated from CML patients.

Figure 8

(A) Expression levels of miR-29a-3p, miR-494-3p and miR-660-5p 24 hours after the last nucleofection as evaluated by qRT-PCR. Data are reported as RQ mean ± S.E.M of 3 independent experiments. Results of Annexin V/PI staining on Lin-CD34-CD38− (B) and Lin-CD34+CD38− (C) cells isolated from CML patients after 48 h of 5 μM IM, or 200 nM Dasatinib or 20 nM Ponatinib treatment (mean ± SEM; n = 3) *p < 0.05, **p < 0.01. Abbreviations: RQ, Relative Quantity; IM, imatinib mesylate; Das, Dasatinib; Pon, Ponatinib; NT, Not Treated; 48 h, 48 hours; PI, Propidium Iodide.