Table 2.
Integrated kinetochore fluorescence for various experimental protocols
| A | Control | 30-min Noc | 30-min Noc, washout | 4-h Noc | 4-h Noc, washout |
|---|---|---|---|---|---|
| CREST | |||||
| n | 63 | 56 | 63 | 60 | 63 |
| mean ± SD | 2753 ± 837 | 3463 ± 1005 | 3132 ± 886 | 1612 ± 404 | 1851 ± 514 |
| ratio to control | 1 | 1.3 | 1.1 | 0.6 | 0.7 |
| BubR1 | |||||
| n | 51 | 53 | 51 | 52 | 50 |
| mean ± SD | 8792 ± 3161 | 33456 ± 7530 | 6794 ± 2846 | 29500 ± 6548 | 6834 ± 2948 |
| ratio to control | 1 | 3.8 | 0.8 | 3.4 | 0.8 |
| CENP-E | |||||
| n | 65 | 57 | 60 | 53 | 63 |
| mean ± SD | 23250 ± 6685 | 57026 ± 15865 | 17807 ± 5275 | 89049 ± 17746 | 27563 ± 6886 |
| ratio to control | 1 | 2.5 | 0.8 | 3.8 | 1.2 |
| Dynein | |||||
| n | 54 | 53 | 50 | 53 | 53 |
| mean ± SD | 120 ± 416 | 6629 ± 3238 | 286 ± 464 | 13182 ± 5713 | 120 ± 254 |
| ratio to control | 1 | 55 | 2.4 | 110 | 1.0 |
| Mad2 | |||||
| n | 53 | 56 | 52 | 66 | 52 |
| mean ± SD | 125 ± 235 | 8030 ± 1978 | 138 ± 404 | 12365 ± 1641 | 3 ± 175 |
| ratio to control | 1 | 65 | 1.1 | 99 | 0.03 |
| B | Control | 45 min Taxol | |||
| BubR1 | |||||
| n | 56 | 51 | |||
| mean ± SD | 16624 ± 4679 | 15932 ± 5344 | |||
| ratio to control | 1 | 1.0 | |||
| CENP-E | |||||
| n | 56 | 57 | |||
| mean ± SD | 23346 ± 6153 | 26972 ± 8371 | |||
| ratio to control | 1 | 1.2 | |||
| Dynein | |||||
| n | 55 | 57 | |||
| mean ± SD | 1624 ± 511 | 1537 ± 575 | |||
| ratio to control | 1 | 1.0 | |||
| Mad2 | |||||
| n | 75 | 67 | |||
| mean ± SD | 1557 ± 7818 | 1003 ± 3191 | |||
| ratio to control | 1 | 0.6 | |||
| C | Control | High Ca2+ Buffer | |||
| BubR1 | |||||
| n | 61 | 51 | |||
| mean ± SD | 6568 ± 1769 | 6943 ± 2095 | |||
| ratio to control | 1 | 1.1 | |||
| CENP-E | |||||
| n | 57 | 53 | |||
| mean ± SD | 10216 ± 2759 | 11034 ± 3188 | |||
| ratio to control | 1 | 1.1 | |||
| Dynein | |||||
| n | 53 | 51 | |||
| mean ± SD | 1187 ± 346 | 906 ± 309 | |||
| ratio to control | 1 | 0.8 |