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(A) Jurkat cells were incubated with 2.5 μM Gal-3 for 10 min, 1 h, 6 h and 18 h and apoptosis was analyzed by PI/FITC-AnnexinV double staining and flow cytometry. (B) Gal-3-treated Jurkat cells were analyzed for the presence of phosphorylated and non-phosphorylated forms of ERK1/2, JNK and p38 MAPKs by western blotting. Also, full length (pro-Casp-3) and cleaved caspase-3 (Cl-Casp-3) were analyzed by western blotting.
