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. 2017 May 10;8(30):49824–49838. doi: 10.18632/oncotarget.17760

Figure 4. Gal-3 triggers apoptotic signaling in different T leukemia cell lines and PBMCs.

Figure 4

(A) Jurkat E6-1, CEM and human PBMC were incubated with Gal-3 in the presence or absence of U0126 for 18 h followed by analysis for p-ERK1/2 and cleaved caspase-3 and (B) p-PKC by western blotting. (C) Jurkat E6-1, CEM and human PBMCs were incubated with Gal-3 for 6 h and ROS production was detected by flow cytometry. (D) Jurkat E6-1, CEM and human PBMCs were incubated with Gal-3 for 18 h in the absence or presence of NAC and AEB071 followed by analysis of p-ERK1/2 and cleaved caspase-3 by western blotting.