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Jurkat cells were treated with 2.5 μM Gal-3 or its variants for 18 h, and (A) ROS production was assessed by flow cytometry and (B) p-PKC was detected by western blotting. (C) Jurkat cells were treated with 2.5 μM G69-250 in the absence or presence of AEB071 or NAC for 18 h and analyzed for p-ERK1/2 and cleaved caspase-3. The data are representative of three independent experiments.
