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. 2017 Jun 1;8(30):49959–49972. doi: 10.18632/oncotarget.18326

Figure 8. Effect of PGF2α and FGF-1 on PGF2α-FP receptor antagonist or ASA-induced effects on the growth of melanoma cells.

Figure 8

(A) B16-MSCV cells were treated with or without various doses of PGF2α-FP receptor antagonist, AL8810 (0.5, 1, 2.5, 5, 10, 25, 50, 75, 100, 200, 300, 350, 400 and 500 μM) and cultured for 72 hour. (B) B16-MSCV cells were pretreated with PGF2α (1.0 μg/ml) or FGF-1 (10 ng/ml) and left for 24 hour followed by treatment with or without AL8810 (0.5 μM and 300 μM) and ASA (5 mM). In both these experiments, control cells received 0.1% DMSO only and after 72 hour, % cell survival was assessed by SRB assay as described. The data are mean ± SD of three independent experiments and expressed as % cell survival over various doses of AL8810 or various groups. The statistical significant differences (P<0.05) were noted between ASA and PGF2α + ASA and FGF-1 + ASA groups (denoted by $), and AL8810* (0.5 μM) and AL8810* + ASA groups (denoted by $) and between AL8810 (300 μM) and PGF2α + AL8810 and FGF-1 + AL8810 groups (denoted by #). (C) The schematic representation of working model is shown.