Figure 9.

EM localization of E8R. HeLa cells were infected with vv and fixed at 3 h postinfection (B), or infected and lipofected with E8R-GFP and fixed at 4 h postinfection (A and C). Sections were labeled with affinity purified anti-E8R (B) or anti-GFP (A and C; anti-GFP or anti-E8R labeling is indicated with small arrowheads). In all three images the factory region is indicated with a star. In A labeling is observed on the inner ER membrane and in ER membranes in continuity with the membranes enclosing the factory region. In B cells were infected for 3 h and labeled with anti-E8R. The image shows one entire replication site and part of a second site in the top right of the picture. Labeling is found on the ER membrane enclosing the DNA site as well as on the inside of the factory region (see text). C shows a part of a factory region of which the inner membrane is labeled. The image also shows an unlabeled Golgi stack (G) as well as the NE. The large arrowhead indicates the electron-dense structure underlying the INM. Nu, nucleus. Bars, 200 nm.