Fig 5. Analysis of propidium iodide uptake of H. somni following incubation with bovine NK2A peptide.

H. somni was incubated with propidium iodide (PI) for 5 min followed by addition of different concentrations of NK2A peptide (1, 2, 5 and 20 μM final concentrations) and PI fluorescence was immediately read using a fluorescent microplate reader. Ethanol-killed and 1% (v/v) Triton X 100 treated H. somni were used as positive controls and untreated and kanamycin (50 μg/ml) treated H. somni were used as negative controls. Error bars indicate standard deviations of the means.