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. Author manuscript; available in PMC: 2017 Aug 21.
Published in final edited form as: Exp Hematol. 2016 Mar 22;44(6):502–507. doi: 10.1016/j.exphem.2016.02.011

Figure 2. Phenotypical and functional characterization of culture-derived FL, UCB, and PBSC CD34+ subpopulations.

Figure 2

(A–C) Sort-purified FL-, UCB-, and PBSC-derived HSC/MPP, LMPP, EMP, and GMP subpopulations (post sort, upper row) were cultured in StemSpan supplemented with SCF, TPO and Flt3-L (100ng/ml each). Phenotype, composition, and proliferation of arising progeny was analyzed on day 3 (post culture, lower row). (D–F) On day 3 of culture, HSC/MPP-derived CD133/CD45RA-subpopulations were sort-purified, introduced into colony-forming cell assay and ability to form different colony-subtypes quantified (all statistics mean ± SEM; *: p<0.05, **: p<0.01, ***: p<0.001).