Fig 5. The altered architecture of ndt80Δ/NDT80 is strongly dependent on Rob1 and Tec1.

(A) Complementation of ndt80Δ/NDT80 with an allele under endogenous promoter restores wild type levels of normalized biofilm density (NBD) NBD=OD₆₀₀ mutant/OD₆₀₀ wild type. The blue bar indicates statistically significant increase relative to WT. Grey bars indicate no difference from WT. (B) The increased biofilm density of ndt80Δ/NDT80 is dependent on other network TFs with Tec1 and Rob1 having the most dramatic effects. The red bars indicate statistically significant reduction relative to WT and ndt80Δ/NDT80 density. (C) Overexpression of TEC1 with the strong constitutive promoter TDH3 partially restores biofilm formation to the ndt80Δ/Δ mutant. Asterisk indicates statistically significant difference between means for strains with and without pTDH3-TEC1 allele as determined by Student’s t test with significance level set at P<0.05.