Fig 5. Myeloid effector mechanisms by Bl-Eng-2.
Mice received 1807 cells prior to vaccination and were vaccinated and boosted with indicated adjuvants and formulated calnexin. Two weeks after the boost, mice were challenged i.t. with 105 DsRed yeast and lungs were harvested 3 days later. The percentage of dead (DsRed-Uvitex+)(blue) yeast among total neutrophil-associated yeast (all Uvitex+ events)(blue and red together) (see gating strategy in S6A Fig) were analyzed and calculated (dot plots are concatenates from 5 mice/group) to depict the amount of killing by neutrophils (A+B). The percentage of killing by alveolar macrophages is shown in (C). The number of live yeast was depicted by showing the total number of DsRed+ events (D) or plating lung CFU (E). The numbers indicate the n-fold reduction in live yeast (DsRed+ or CFU) vs. the calnexin control groups. *p<0.05 control groups without Bl-Eng2. Cnx denotes calnexin.