Figure 2.

Newly formed acetylated MTs associated with Golgi elements are stable. Nocodazole wash-out experiments were conducted as described in Figure 1 on Fao (A and C) and WIF-B cells (B and D). Cells were either permeabilized with HEPES-buffered medium containing 0.2 mg/ml saponin (A, B) or incubated again with 10 μM nocodazole for 20 min at 37°C (C and D). After fixation, cells were subjected to double immunofluorescence labeling of acetylated tubulin (green) and secretory albumin (red). All cells were examined by confocal microscopy. The images are the superimposition of consecutive optical sections taken over the whole cell height (delta z = 0.5 μm). Scale bar, 10 μm.