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. Author manuscript; available in PMC: 2018 May 2.
Published in final edited form as: Biochemistry. 2017 Apr 21;56(17):2315–2327. doi: 10.1021/acs.biochem.7b00103

Figure 2.

Figure 2

Regulation of gene expression. (A) Relative transcript level of ecsC, emrB, and bif R in wild type (WT) strain and ecsC and emrB in bif RΔ and bif RΔ e-b strain (complemented with emrB-bif R) calculated using 2−ΔCT relative to reference gene encoding glutamate synthase large subunit (BTH_I3014/BTH_RS27550). Error bars represent standard deviation of three separate experiments (each with three technical replicates). Asterisks represent statistical significance from WT based on Student’s t test (p < 0.001). (B) Elastin cleavage activity in WT and emrBΔ-bif RΔ strains and emrBΔ-bif RΔ complemented with emrB (emrBΔ-bif RΔ e) or with emrb-bif R (emrBΔ-bif RΔ e-b); WT c carries vector without genes inserted. Plates contain insoluble elastin and clearing zone reflects elastin cleavage.