Table 1.
Small Molecules Identified by Chemical Screening in Zebrafish for Suppressors of PAA Angioblast Differentiation
| Category | Compounds |
|---|---|
| Kinase and phosphatase inhibitors | |
| TGF-β receptor inhibitor | CB 41227199a |
| Protein kinase A and B inhibitor | CB 97929926 |
| Protein kinase C inhibitor | calphostin C |
| Thymidine kinase inhibitor | CB 17846240 |
| Cyclin-dependent kinase inhibitors | CB 42005171, CB 20372475, CB 14126598 |
| IκB kinase inhibitors | CB 15143266, CB 28324237 |
| c-Jun N-terminal kinase 3 inhibitor | CB 34431373 |
| ERBB2 kinase inhibitor | tyrphostin AG-825 |
| RAF kinase inhibitor | CB 14171361 |
| SRC kinase inhibitor | CB 14280993 |
| CD45 phosphatase inhibitor | RWJ-60475-(AM)3 |
| Bioactive lipids, modulators of lipid metabolism or signaling | |
| Omega-3, 6, 9 fatty acids | eicosatrienoic acid (20:3 n-3) (11,14,17-ETE) docosahexenoic (22:6, n-3) (4,7,10,13,16,19-DHA) arachidonic acid (20:4, n-6) (5,8,11,14-AA) eicosatriynoic acid (20:3, n-9) (5,8,11-ETI) |
| Cyclooxygenase inhibitors | indomethacin, indoprofen |
| Lipoxygenase metabolite | 12(R) = HETE |
| Prostaglandins | PGD2; 13,14-Dihydro-PGE1; 17-phenyl-trinor-PGE2; 9B,11A-PGF2 |
| Leukotriene B4 antagonist | U-75302 |
| Ion channel modulators and ionophores | |
| Potassium channel openers | cloxyquin, minoxidil, rottlerin |
| Calcium ionophore | ionomycin |
| Nuclear hormone receptor inhibitors | |
| Liver × receptor inhibitor | CB 78238822 |
| Retinoic acid receptor inhibitors | CB 70700976, CB 82029493 |
| Other signaling modulators | |
| Nuclear factor κB (NF-κB) inhibitor | caffeic acid phenethyl ester |
| Adenosine A3 receptor agonist | IB-MECA |
| Phosphodiesterase inhibitors | zardaverine, dyphylline |
| Histamine H1 receptor antagonists | doxylamine succinate, carbinoxamine maleate |
| Antimicrobials | fusidic acid, chloroxylenol, nitrofurantoin |
| Other | N-Acetyl-S-geranyl-L-cysteine, guanethidine sulfate, roxarsone, glucosamine hydrochloride, guaifenesin, lactulose, alrestatin |
Four compound libraries were screened, and hits (right column) were binned into categories (left column) based on biological activities.
a
One hit, CB 41227199, highlighted the importance of TGF-β signaling in PAA angioblast differentiation.