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Expression of nephrin in DN rats of different ages. (A) The protein nephrin determined by immunohistochemistry. (a–c) Nephrin expression of DN four-week rats, eight-week rats, and 12-week rats. (B) The expression of nephrin determined by Western blot assay. (C) Analysis of the result B. * p<0.05, compared with 4-week rats. Bar: 50 μm.
