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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Signal. 2017 Feb 24;34:86–91. doi: 10.1016/j.cellsig.2017.02.021

Figure 3. Gemcitabine induces ASMase secretion.

Figure 3

A: 15 minutes after adding gemcitabine to proliferating BAEC, medium was harvested and concentrated using a 30 kDa molecular weight cutoff spin column. ASMase activity (sphingomyelin conversion, nmol/mg/h) in concentrated medium samples was determined in the absence (open bars) or presence (grey bars) of exogenous zinc. Activity of the secretory ASMase is dependent on exogenous zinc. B: The rate of ASMase activity secreted per minute is linearly related to gemcitabine concentration. Baseline ASMase secretion rate (0 μM) was subtracted from the gemcitabine-stimulated samples. Data (mean ± SEM) are collated from 5 experiments.