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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Signal. 2017 Feb 24;34:86–91. doi: 10.1016/j.cellsig.2017.02.021

Figure 4. Gemcitabine activates and induces secretion of ASMase exclusively in proliferating HCAEC.

Figure 4

A: Total cellular ASMase activity in proliferating HCAEC upon addition of gemcitabine (10 μM). B: Stable ASMase activity profile upon gemcitabine addition to HCAEC, growth-arrested by 3 days confluence. C: Measurement of ASMase activity secreted into the medium. Gemcitabine induces time-dependent secretion of ASMase in proliferating HCAEC. D: ASMase secretion from growth-arrested HCAEC. Medium was collected and cells were lysed at the indicated time points after gemcitabine (10 μM) addition. Data (mean ± SEM) are collated from 4 separate experiments.