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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Signal. 2017 Feb 24;34:86–91. doi: 10.1016/j.cellsig.2017.02.021

Figure 5. Proliferating endothelial cells are gemcitabine sensitive, in contrast to growth-arrested cells.

Figure 5

Endothelial cells were either proliferating (closed circles), or confluent at growth-arrest (open circles). A: BAEC were exposed to gemcitabine and MTT conversion was measured as a readout for cell viability. Cells treated with vehicle as a control were set at 100%. B: After 16 hours gemcitabine exposure, BAEC cells were harvested and apoptosis was quantified by bis-benzimide nuclear staining. C: Pre-treatment (45 min) of BAEC with the pan-caspase inhibitor z-VAD-fmk blocks apoptosis induced by 10 μM gemcitabine. D: HCAEC were exposed to gemcitabine for 16 hours and apoptosis was quantified. Data (mean ± SD) are collated from 3 separate experiments each.