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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Signal. 2017 Feb 24;34:86–91. doi: 10.1016/j.cellsig.2017.02.021

Figure 6. Pharmacologic ASMase inhibition and anti-ceramide antibody prevent gemcitabine-induced apoptosis.

Figure 6

A: Pre-incubation with up to 150 μM imipramine inhibits gemcitabine-induced endothelial apoptosis. Imipramine was added to proliferating BAEC 30 minutes before 1.0 μM gemcitabine. B: Anti-ceramide monoclonal antibody blocks gemcitabine-induced apoptosis in proliferating endothelial cells. BAEC were pre-incubated with anti-ceramide antibody for 15 min prior to 1.0 μM gemcitabine. Cells were harvested after 16 hours and apoptosis was quantified by bis-benzimide staining. Data (mean ± SD) are collated from 3 separate experiments each.