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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Cell Signal. 2017 Feb 24;34:86–91. doi: 10.1016/j.cellsig.2017.02.021

Figure 7. Exogenous sphingomyelinase restores gemcitabine-induced apoptosis in confluent BAEC.

Figure 7

A: Bacterial sphingomyelinase (Bacillus cereus; bSMase) was applied with or without 1 μM gemcitabine to a confluent monolayer of BAEC in a 2 cm2 well. Apoptosis was quantified after 16 hours. Growth-arrested BAEC otherwise resist gemcitabine (leftmost bar). B: Anti-ceramide antibody blocks the apoptosis, induced by bSMase plus gemcitabine on confluent BAEC. Anti-ceramide antibody (10 μg/ml) was added prior to bSMase (5 units) and gemcitabine (1 μM) treatment. Asterisk denotes a p value < 0.05. The activity of a bSMase unit is defined by the hydrolysis of 1.0 μmol sphingomyelin per minute at 37°C. Data (mean ± CV) are collated from 3 separate experiments each.