Figure 2. Cytonemes from A and P compartment cells interact during Hh reception.
(A) 3D view of a ptc.Gal4, tub.Gal80ts>UAS.shiK44A wing disc after 12 hr at the restrictive temperature and double stained for Hh and Ptc. Note that Ptc colocalizes with Hh at the basal membranes of the Hh-receiving cells when endocytosis is frozen using a dominant negative form of Dynamin (arrowheads). (A’) Transverse section of an extracellular staining for both Hh and Ptc in a similar wing disc. Note the colocalization of Hh and Ptc in the most basal part of the disc (arrows). (a’) Magnification of A’. (B) 3D view of a ptc.Gal4, tub.Gal80ts>UAS.shiK44A / hh.LexA>LexAop.ihog-RFP wing disc after 12 hr at the restrictive temperature and labelled with α-Ptc antibody. Note Ihog-RFP localization in the P compartment cytonemes and Ptc in the A compartment cytonemes. (B’) Transverse section of a similar wing disc stained for extracellular Ptc. Note the colocalization of Ihog and Ptc in the most basal part of the epithelium (arrows). (b’) Magnification of B’. (C, C’) Basal (C) and transverse (C’) sections of a ptc.Gal4, tub.Gal80ts>UAS.ihog-RFP>UAS.shiK44A wing disc after 12 hr at the restrictive temperature and stained with α-Ptc antibody. (c’) Magnification of C’. Note that when endocytosis is frozen, Ptc is located in A compartment cytonemes labelled with Ihog-RFP (arrow).
Figure 2—figure supplement 1. Cytonemes from A and P compartment cells interact during Hh reception.
