Video 1. Dynamics of filopodia of A compartment abdominal histoblast cells expressing only GMA and co-expressing Ihog.
(A-B) Abdominal histoblasts of pupae with the genotype w; ptc.Gal4 / tubGal80ts; UAS.GMA-GFP /+ (A) and w; ptc.Gal4 / tubGal80ts; UAS.GMA-GFP / UAS.ihog-RFP (B). The actin-binding domain of moesin fused to GFP (GMA-GFP) was expressed during 24 hr in Hh-receiver cells to visualize actin-based filopodia dynamics, easily detected using the inverted grey-scale lookup table tool of Fiji (A). Notice the highly dynamic filopodia emerging from A cells towards P cells. GMA-GFP (B) was co-expressed with Ihog-RFP (B’) in Hh-receiver cells. Observe in the merge panel (B’’) that Ihog-containing filopodia are stabilized while few filopodia with low or no Ihog levels detected are more dynamic. Histoblasts move up (A) and down (B) towards the dorsal midline, not shown because of the high magnification. Anterior is on the left. Pupae were around 30 hr APF (after puparium formation). Movies of 30 min imaging with time intervals between frames of 2 min. Scale bars represent 10 μm.