Video 7. Filopodia and ptc mutant region manual tracking using MTrackJ.
(A-C) Abdominal histoblasts of a pupae with a y w hs-Flipase122; FRT42D ptc16 / FRT42D ubiRFPnls; hh.Gal4 / UAS.GMA-GFP genotype. GMA-GFP expressed during all development and two days ptc16 clones (induced by heat-shock of 1 hr at 37°C). (A) Z-projection of GMA-GFP using the inverted grey-scale lookup table of ImageJ. (B) Z-projection of GMA-GFP and the tracking of filopodia using the MTrackJ plugin of Fiji, where the fist number refers to the number of filopodium tracked and the second name refers to the base (1) or the tip (2). Each filopodium has a colour and the coloured lines are the base and tip trajectories. Notice that also two dying larval epithelial cells are tracked (orange tracks #165.1 and #165.2). (C) Nuclear RFP (magenta) wild-type nuclei and FRT42D ptc16 mutant clone (absence of magenta) tracked using the first and last region where there is no RFP-positive nuclei just anterior to the A/P boundary (blue tracks #164.1 and #164.2). Histoblasts move down towards the dorsal midline, and this is not shown because of the high magnification. Anterior is on the left. Pupa is around 30 hr APF (after puparium formation). Movie of 30 min imaging with a time interval between frames of 30 s. Scale bars represent 10 μm.