Fig. 5.

MeCP2 KO selectively alters DRG genes regulating actin dynamics and neuritogenesis. (A) Heat map analysis of counts obtained from mapped reads shows that samples cluster by genotype. (B) Plot of RNA-Sequencing fold-changes vs. qPCR fold-changes of multiple candidate genes reveals a strong correlation (r² = 0.89, P < 0.001). (C) Comparison of number of differentially expressed genes in DRG with that reported in the literature for brain regions that also used ± 1.2 fold-change and 0.05 false-discovery rate (FDR) cut-off. (D) IPA identified major canonical pathways affected by MeCP2 disruption in sensory neurons (x axis = negative log to base 10).