Fig. S8.

Effect of shRNA knockdown mixture in primary DRG neurons. (A) Efficiency of individual shRNAs in the KD-Mix was established in 50B11 cells using q-PCR. (B) Mecp2^−/y primary DRG neurons show profuse outgrowth when cultured in the presence of scrambled (Scr) lentiviral shRNA (Scale bar, 50 µm.). (C) Neurite outgrowth appears reduced in cultures containing the lentiviral shRNA knockdown mixture (KD-Mix). (D) Analysis shows that outgrowth per neuron is reduced upon knockdown of MeCP2-regulated downstream candidate genes (*P = 0.012, n = 4 per group; neurons from four animals transduced separately with either scrambled or knockdown mixture).