Fig. S9.

Down-regulating key cytoskeletal genes has no appreciable effect on neuroanatomical and behavioral phenotypes in WT rats. (A) Following lentiviral shRNA mixture injection into the hind paw, withdrawal threshold to mechanical stimulation was not significantly different from that seen after scrambled shRNA control paw injection. (B) No change was also observed percent withdrawal to a fixed mechanical force. (C) Footpad injected with knockdown shRNA did not elicit a significant change in heat sensitivity. (D) Quantitation of PGP9.5 innervation in the footpad revealed no change in IENF per millimeter following knockdown mixture injection. (E) Quantitation showed no appreciable change of peptidergic innervation after knockdown injection. n = 6 WT rats. Data in bar graphs are presented as mean ± SEM.