Fig. S2.

Dia1 and Dia2 do not regulate MT stabilization and infection by nonhuman retroviruses. (A) CHME3 cells were left noninfected (NI) or infected with mock virus, MuLV–VSV–Luc or SIV–VSV–Luc. Then samples were fixed at the indicated hours post infection (h.p.i.) and stained for tyrosinated tubulin (Tyr-MTs) and acetylated tubulin (Ac-MTs) MTs. (Scale bar, 40 μm.) Similar results were obtained in at least three independent experiments. (B and C) CHME3 cells were transfected with siRNA targeting Dia1, Dia2, or negative control (NC). Cells were either lysed for WB to verify knockdown using antibodies against Dia1, Dia2, and GAPDH (as loading control) (B) or infected with MuLV or SIV virus carrying a luciferase reporter pseudotyped with VSVG envelope (C). Levels of infection were determined by measurements of luciferase activity. Data are shown as scatterplot with mean, n = 3. (D and E) CHME3 cells were treated with DMSO (0.1%) or 10 μM SMIFH2, and infected with (D) MuLV or (E) SIV virus carrying a luciferase reporter pseudotyped with VSVG envelope. Levels of infection were determined by luciferase assay. Similar results were obtained in four independent experiments. Data are shown as scatterplot with mean, n = 4.