Fig. S4.

Electrostatic potentials and conservation of Cc ProXp-ala. (A) Electrostatic potentials of Cc ProXp-ala plotted on the molecular surface (PDB ID code 1VJF) as a gradient from red (−3.0 kT) to white (0 kT) to blue (3.0 kT). Potentials were calculated using the adaptive Poisson–Boltzmann solver (APBS) plugin within PyMOL. (B) Normalized conservation score within all Bacteria of Cc ProXp-ala plotted on the structure (PDB ID code 1VJF) as a gradient from orange (−1) to white (0) to green (3). Scores were determined from the AL2CO webserver (prodata.swmed.edu/al2co/al2co.php). (C) Graph of normalized conservation scores used to generate the map in B. The highly conserved residues mentioned in this study are labeled in B and C.