Fig. 5.

Eta is nonessential, is not responsible for polarity in vivo, but loss of Eta limits growth and increases DNA damage susceptibility. (A) Deletion or inactivation of Eta hinders growth rate and final cell densities. The curves and errors shown represent means and standard averages of triplicate technical repeats of triplicate biological samples. (B) Eta is not responsible for polarity in vivo. The presence or absence of the nonsense codon in PF1848 is noted, and the percentage of β-glycosidase activity is reported as the mean and standard average of triplicate technical repeats of triplicate biological samples. (C) Deletion of Eta increases sensitivity to DNA damaging agents.