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Eta purification and in vitro activity of EtaΔ1–193. (A) Linear representation of Eta highlighting the N-terminal domain (green), the P-loop NTPase domain (gray), and the Walker A and Walker B motifs (blue and orange, respectively). (B) Recombinant Eta and Eta variants (EtaD344A/E345A and Eta ∆1–193) were purified for use in vitro. Mass standards (M) are shown in kDa. (C) Eta ∆1–193 retains termination activity in vitro.
