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Purified Eta and RNAP do not retain long-lived interactions in vivo. A pull-down experiment was performed using a nickel resin and RNAP-his6 only (positive control), Eta only (negative control), and RNAP-his6 + Eta. The portion of the sample that did not stick to the resin is indicated as flow-through, and the portion of the sample that eluted when a high imidazole buffer was added is indicated as elution. Samples were resolved on an SDS/PAGE with a marker shown in kDa.
