Table S2.
Plasmids and T. kodakarensis strains used in this study
| Plasmid or strain | Key features | Source |
| Plasmids | ||
| Transcription templates | ||
| pTS236 | PhmtB with 376 nt G-less cassette (450 nt run off transcript) | Ref. 22 |
| pJW21 | PhmtB with 58 nt C-less cassette (128 nt run off transcript) | This work |
| pJW32 | PhmtB with 20 nt C-less cassette (128 nt run off transcript) | This work |
| pJW29 | PhmtB with 58 nt C-less cassette and Ssp1 cut site at position +18 (128 nt run off transcript) | This work |
| pJW30 | PhmtB with 58 nt C-less cassette and Ssp1 cut site at position +37 (128 nt run off transcript) | This work |
| Protein expression vectors | ||
| pTS317 | pQE-80L-TK0566 | This work |
| pJW26 | pQE-80L-TK0566D344A/E345A | This work |
| pJW28 | pQE-80L-TK0566∆1–193 | This work |
| Strain construction | ||
| pCSU-TK0566B | pTS700 containing TK0565:TK0567 | Ref. 45, this work |
| pOSU-TK0566NT | pTS700 containing TK0565-(his6-HA)TK0566-TK0567 | This work |
| pCSU-TK0566D344A/E345A | pCSU-TK0566B with D344A and E345A | This work |
| pTS429 | PhmtB (PF1848–TK1761, TK1762, TK1763) with adjacent trpE selectable marker | Ref. 32 |
| pTS435 | pTS429 with a stop codon at amino acid 381 of PF1848 | Ref. 32 |
| T. kodakarensis strains | ||
| TS559 | ΔTK2276, ΔTK0254::TK2276, ΔTK0664, ΔTK0149 | Ref. 45 |
| ∆TK0566 | TS559 ΔTK0566 | This work |
| TK0566-NT | TS559 TK0566-N-terminal his6-HA tag | This work |
| TK0566D344A/E345A | TS559 TK0566D344A/E345A | This work |