Thermotolerance and lifespan. (A) WT and cyp-37A1(ssd9) 1-d adult animals started to die upon 2-h treatment of 34 °C. All animals died upon 8-h treatment. (B) Under a 34 °C/4-h treatment, the survival rate of cyp-37A1(ssd9) is higher than that of WT. The better survival of cyp-37A1(ssd9) is partially impaired by daf-12(m583) mutation. Data are mean ± SEM of six replicates. ***P < 0.001, *P ≤ 0.05, unpaired two-sample t test. (C) At 20 °C, the lifespan of cyp-37A1(ssd9) is slightly increased compared with that of WT. The lifespan increase of cyp-37A1(ssd9) is abolished by daf-12(ssd155) mutation. (D) At 25 °C, the lifespan of cyp-37A1(ssd9) is much increased than that of WT. The lifespan increase of cyp-37A1(ssd9) is abolished by daf-12(ssd155) mutation. Lifespan results are typical of two independent experiments.