Table 1.
Projection parameters for each target area
| Nucleus | Coordinates in mm from bregma | Interquartile summary statistics | Bouton diameter, µm | Boutons per L5B neuron | Boutons per column | Volume per L5B neuron, 10−4 mm3 | Volume per column, 10−4 mm3 | Bouton density, 10−4/mm3 | Soma density, 10−4/mm3 | Boutons per soma | Overlap, % | Map orientation (L/A/V) | |||||
| L | P | V | GFP | mO | Rows (A–E) | Arcs (1–6) | |||||||||||
| POa | 1.0 | 1.3 | 3.2 | Median | 2.58 | 2.86 | 5.8 | 263 | 0.6 | 24.4 | 7.0 | 3.26 | 2.2 | 60.3 | L | −0.23 | −0.16 |
| Q1 | 2.33 | 2.56 | 3.4 | 120 | 0.4 | 18.0 | 6.0 | 2.54 | 1.9 | 26.7 | A | −0.59 | -0.99 | ||||
| Q3 | 2.95 | 3.15 | 16.0 | 384 | 1.3 | 47.1 | 12.3 | 4.69 | 3.8 | 63.6 | V | 0.70 | 0.02 | ||||
| POmlateral | 1.4 | 2.0 | 3.3 | Median | 2.88 | 3.20 | 24.4 | 1,027 | 1.1 | 32.0 | 36.5 | 4.50 | 8.1 | 18.7 | L | −0.21 | −0.82 |
| Q1 | 2.56 | 2.86 | 16.7 | 707 | 0.6 | 25.3 | 18.4 | 3.90 | 4.1 | 13.2 | A | 0.38 | 0.55 | ||||
| Q3 | 3.40 | 3.65 | 53.3 | 1,551 | 1.6 | 47.2 | 47.0 | 4.86 | 10.4 | 23.0 | V | 0.90 | −0.14 | ||||
| POmmed | 0.8 | 1.7 | 3.2 | Median | 2.64 | 2.88 | 6.0 | 223 | 0.5 | 15.4 | 14.9 | 4.50 | 3.3 | 21.0 | L | −0.45 | 0.94 |
| Q1 | 2.33 | 2.58 | 3.3 | 140 | 0.2 | 10.9 | 12.2 | 3.58 | 2.7 | 17.8 | A | 0.26 | 0.30 | ||||
| Q3 | 3.04 | 3.26 | 9.1 | 265 | 0.7 | 29.3 | 24.5 | 5.30 | 5.4 | 26.5 | V | 0.86 | 0.15 | ||||
| PoT | 0.9 | 2.3 | 3.3 | Median | 2.56 | 2.73 | 9.3 | 319 | 1.7 | 63.9 | 3.5 | 4.89 | 0.7 | 43.9 | L | −0.20 | 0.63 |
| Q1 | 2.24 | 2.44 | 2.9 | 95 | 0.6 | 26.9 | 2.3 | 3.53 | 0.5 | 32.9 | A | 0.58 | 0.73 | ||||
| Q3 | 2.88 | 3.15 | 13.8 | 399 | 3.7 | 118.9 | 6.9 | 5.56 | 1.4 | 64.9 | V | 0.79 | 0.27 | ||||
| ZI | 1.3 | 2.4 | 4.3 | Median | 2.31 | 2.58 | 17.9 | 483 | 5.0 | 161.0 | 2.6 | 2.23 | 1.2 | 58.3 | L | −0.93 | −0.34 |
| Q1 | 2.05 | 2.31 | 4.6 | 223 | 3.0 | 109.1 | 1.6 | 1.33 | 0.7 | 47.0 | A | 0.02 | 0.43 | ||||
| Q3 | 2.58 | 2.86 | 28.3 | 557 | 13.7 | 263.8 | 3.6 | 2.65 | 1.6 | 75.0 | V | 0.36 | 0.83 | ||||
| APT | 0.8 | 2.7 | 3.1 | Median | 2.44 | 2.64 | 29.5 | 1,003 | 2.2 | 67.7 | 9.2 | 4.31 | 2.1 | 29.8 | L | −0.44 | 0.94 |
| Q1 | 2.24 | 2.44 | 10.7 | 415 | 0.7 | 30.3 | 7.9 | 1.85 | 1.8 | 25.2 | A | −0.74 | −0.21 | ||||
| Q3 | 2.73 | 2.95 | 43.5 | 1,551 | 3.7 | 131.0 | 19.8 | 6.02 | 4.6 | 34.2 | V | 0.50 | 0.28 | ||||
Summary of parameters of L5B projections to the six target nuclei in thalamus and anterior midbrain with medians (bold) and first (Q1) and third (Q3) quartiles, where applicable. Coordinates are equivalent to the mouse brain atlas (12). Diameters are affected by the fluorophore [GFP or mOrange (mO)]. Map orientations are given as unit vectors in the cardinal directions: lateral (L), anterior/posterior (A/P), and ventral (V). Procedures of calculations are given in SI Materials and Methods.