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Enhancement of TAM-mediated efferocytosis by Tim4. TKO, TKO-mAxl, TKO-mMer, and TKO-mTyro3, and their transformants expressing mTim4 were incubated with pHrodo-apoptotic thymocytes in DMEM containing 5 mg/mL BSA and the indicated concentrations of mProS or mGas6, and analyzed by flow cytometry. Experiments were performed in triplicate, and the average percentage of pHrodo+ cells was plotted with SD (bar) as efferocytosis.
