Fig. 5.

Different expression of TAM receptors and Tim4 by tissue macrophages. (A) RNA from mouse skin macrophages (skin mac), Kupffer cells (Kupffer), microglia from glial culture, and thioglycollate-elicited peritoneal macrophages (thio-pMac), was subjected to real-time RT-PCR for Axl, Mer, Tyro3, and Tim4. Each mRNA level is expressed relative to β-actin mRNA level. (B) Cell lysates from the indicated macrophages were analyzed by Western blotting with antibodies against mAxl, mMer, mTyro3, or mTim4. Arrowheads and asterisks indicate specific and nonspecific bands, respectively. (C) Cryosections of liver and skin were stained with antibodies against Tim4 (green) and CD169 (red). (Lower) Merged images. der, dermis; epi, epidermis. (Scale bar, 50 μm.)