Fig. S1.

Recombinant mTAM receptor–Fc, mProS, and mGas6. (A) Preparation of mTAM receptor–Fc proteins, mProS, and mGas6. Fusion proteins between the extracellular region of mTAM receptors (mTyro3, mAxl, and mMer) and human Ig–Fc, and Flag-tagged mProS and mGas6, were produced in 293T cells. The mTAM receptor–Fc fusion proteins were affinity purified using Protein A–Sepharose, whereas the Flag-tagged mGas6 and mProS were purified by anti-Flag M2 Affinity Gel followed by a HiTrap Q column. The purified proteins (2 μg) were subjected to 10% SDS/PAGE and stained with Coomassie Brilliant Blue. The mTim4–Fc was similarly analyzed by SDS/PAGE. Proteins for molecular mass standards were run in parallel, and their molecular masses are shown in kilodaltons. (B) Gel filtration of the recombinant mProS and mGas6. The purified mProS and mGas6 (about 10 μg protein) were analyzed by size exclusion chromatography using a Superdex 200 Increase 3.2/300 gel filtration column. As molecular mass standards, blue dextran, ferritin, aldolase, and conalbumin were similarly analyzed, and the elution positions are indicated with their molecular masses.